Nikon Multiphoton / Super Resolution Imaging System

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Nikon A1RMPsi-STORM4.0 located at the University of Florida McKnight Brain Institute

We are pleased to announce the availability of a Nikon A1RMPsi-STORM4.0 which is located at the University of Florida’s McKnight Brain Institute (MBI). This impressive and versatile system is installed at the Cell and Tissue Analysis Core (CTAC). This multiphoton/super resolution imaging system is supported by the MBI, Office of Research, College of Medicine, and an NIH Shared Instrumentation Grant (1S10OD020026) to Dr. Habibeh Khoshbouei.

This system was purchased with funds from an NIH Shared Instrumentation Grant. The NIH requires that any papers or abstracts that utilize data generated on this system must cite the NIH grant in the acknowledgements. Additionally, we are required to make regular usage reports to the NIH. Therefore, all system users must adhere to the following points:

  1. All system users must accurately and legibly log their time and account information on the provided log sheet.
  2. Any papers or abstracts that utilize images or data generated on this system must cite NIH Grant # 1S10OD020026 in the acknowledgements.
  3. Send a copy of the final published version of any papers, review articles or abstracts that used images or data generated on this system to Doug at dugsmith@ufl.edu .

The Nikon A1RMPsi-STORM4.0 imaging solution is a turnkey system with a number of unique features that will enhance the end-user’s research. Such a turnkey multiphoton and super resolution imaging solution allows UF researchers to 1) image deeper than traditional confocal limitation, 2) reconstruct super resolution structure, and also 3) utilize the system for single-photon confocal imaging.

Some of the capabilities on this impressive and versatile system listed below:

  • High Resolution single-photon excitation confocal imaging
  • Fast confocal imaging with a resonant scanners
  • Confocal hybrid (galvano/resonant scanners) mode for advanced applications such as photostimulation
  • and photoactivation
  • Multiphoton imaging with either or both scanners with a tunable multiphoton laser (700nm-1080nm)
  • TIRF imaging with a Hamamatsu Flash 4.0 sCMOS camera for high resolution and large FOV
  • The new N-STORM 4.0 for super resolution imaging, suitable for both live-cell and fixed imaging, is
  • capable to resolve 20nm in lateral resolution and 50nm in axial resolution

A1RMPsi-STORM4.0 General system description:

  • Visible wavelengths for single-photon and TIRF imaging:
    • LU-NV single laser launch with diode lasers: 405nm(20mW), 445nm(20mW), 488nm(70mW), 515nm(40mW), 561nm(70mW), and 647nm(125mW)
    • 4 channel simultaneously: 405nm/488nm/561/640nm
    • All combinations of wavelengths are possible in sequential mode!
    • Transmitted light detector for a widefield overlay in addition to the fluorescence confocal
  • Multiphoton imaging with a Spectra-Physics Mai Thai DS laser
    • This mode can integrate the 405nm laser for photoconversion!
    • Spectral detector to unmix overlapping fluors
  •  Widefield imaging with the all-LED Lumencor Sola Light Engine (365nm-650nm)
    • Hamamatsu Flash 4.0 sCMOS camera (up to 500 FPS)
    • Filters available:
      • DAPI, CFP, GFP, YFP, DS Red, TxRed, Dual GFP/TxRed, CY5
  • TIRF imaging with LU-NV laser launch
  •  N-STORM 4.0 Super Resolution
    • Hamamatsu Flash 4.0 sCMOS camera (up to 500 FPS)
    • Imaging channels (488/561/647nm
    • Resolution – 20nm lateral, 50nm axial

 Service Contact

Doug Smith: Email / 352.392.1745

Disclaimer: The services and systems offered are available to researchers within the University of Florida system and affiliated institutions, as well as some off-campus entities with NIH funding, for their basic research projects or as part of basic research collaborations with other academic institutions or nonprofit organizations, which do not include involvement or funding from for-profit (commercial) organizations. The CTAC reserves the right to refuse service to any entity or project that may present a biological or health hazard, introduce potential pathogens, or otherwise pose a risk to staff or contamination to the facility.