Olympus IV100 Intravital Microscope

IV100The IV100 intravital laser scanning microscope offers near-confocal resolution thanks to fiber optic laser light delivery, with simultaneous 3-channel acquisition from 488/561/633/748nm excitation lasers.

The core technology – the MicroProbe objectives – allow researchers to do minimal surgery on their animals and yet view the tissue of interest with an objective lens at a cellular level.


Key features of the Olympus IV100 include:

  • 3 simultaneous detection channels for multicolor fluorescence
  • 4 lasers:
    – Argon laser @ 488 nm
    – Diode-pumped solid state (DPSS) laser @ 561 nm
    – Red Helium-neon laser @ 633 nm
    – Diode laser @ 748 nm
  • Immersible Microprobe lens technology for minimally invasive in vivo visualization and simple chemical sterilization
  • Microprobe objectives:
    – 6x: Wide angle, NA 0.14, 13mm diameter lens, large FOV of 670 microns
    – 20x: High resolution, NA 0.5, 1.3mm diameter lens, FOV of 200 microns
    – 27x: High resolution, NA 0.7, 3.5mm diameter lens, FOV of 200 microns
  • Conventional Objectives: 4x, 10x, 20x, 40x
  • Multipositional, tilting scanhead
  • X,Y,Z,T scan combination
    – 76mm range in Z
  • Heated stage, integrated Isoflurane rig.

Service Contact

Gary Brown: Email / 352.273.6569

Disclaimer: The services and systems offered are available to researchers within the University of Florida system and affiliated institutions, as well as some off-campus entities with NIH funding, for their basic research projects or as part of basic research collaborations with other academic institutions or nonprofit organizations, which do not include involvement or funding from for-profit (commercial) organizations. The CTAC reserves the right to refuse service to any entity or project that may present a biological or health hazard, introduce potential pathogens, or otherwise pose a risk to staff or contamination to the facility.