CTAC ICBR SOP: Recognition of the Controls to Customize and Manipulate Images Using the Leica TCS SP5 Confocal Microscope System

Materials Required:

Purpose:

To facilitate recognition of the controls of the Leica TCS SP5 Confocal Microscope System in customizing and manipulating acquired images.

Right side of confocal image screen

Button	Function
	– These buttons allow you to turn different channels of a sequential scan on and off.
	– Overlays all colors to form a single composite image.
	– Takes the multiple images from a Z stack and combines the brightest pixels into one image.
	– Shows the intensity along a chosen X and Y axis through each section of a stack.
	– Permits the user to see each image through a stack while the instrument is optically sectioning.
	– Advances the images of a stack one at a time, all the way to the beginning or end, or play them in sequence as a movie.

Left side of confocal image screen

Button	Function
	– Expands the options so that you have access to all the tools.
	– Highlights items to be moved on the screen or highlighted for other functions.
	– This button highlights all of the annotations that have been added onto an image.
	– Highlighting items and then clicking this button removes the item from the image.
	– Define areas of interest by drawing a line, placing circles / ellipses or squares / rectangles.
	– Place arrows to define points of emphasis.
	– Type in text by hand or place the time and date onto an image.
	– Places a micron bar for size reference on an image. To place a micron bar of a certain length, highlight it after it has been placed on the image, right click and choose “Properties”. You may then enter in the length that you want the bar to be. You may also reposition the bar by clicking and dragging it to another location.
	– Define ROI (Region of Interest) with numbers.
	– Zoom in or out of a captured image.
	– Make images the correct size to avoid being pixelated when saved and printed.

Cell & Tissue Analysis Core