Leica DM2500 Upright Fluorescent Microscope

QImaging Retiga 4000R Monochrome Camera

QCapture Pro 7* (Windows 7)

HBO 103 W/2 Mercury Arc Lamp


  • 1.25x) HCX PL FLUOTAR  (Leica # 11506215)  NA=0.04  WD=3.7mm
  • 5x) HCX PL S-APO  (Leica # 11506288)  NA=0.15  WD=13.7mm
  • 10x) HC PL APO  (Leica # 11506284)  NA=0.40  WD=2.2mm
  • 20x) HC PLAN APO  (Leica # 11506166)  NA=0.70  WD=0.59mm
  • 40x) HCX PLAN APO  (Leica # 11506167)  NA=0.85  WD=0.24mm
  • 63x Water Immersion) HCX PL APO WCORR (Leica # 11506279) NA=1.20 WD=0.22mm
  • 63x Oil Immersion) HCX PL APO  (Leica # 11506187)  NA=1.40-0.60  WD=0.1mm
  • 100x Water Immersion) HCX PL APO  (Leica # 11506220)  NA=1.40-0.70  WD=0.09mm

Fluorescent Filter Sets

  • Blue / DAPI: Leica # A4
  • Green / GFP (Long Pass): Leica # I3
  • Red / Texas Red: Leica # TX2
  • Green – Red Dual Band: Leica # G/R

*For online software tutorials, please see the following:

Basic Operation

Capturing Monochrome Images: Standard fluorescent imaging mode, for taking pseudo-color single and multi-channel fluorescence images, or basic B&W brightfield.

Service Contact

Doug Smith: Email / 352.392.1745

Disclaimer: The services and systems offered are available to researchers within the University of Florida system and affiliated institutions, as well as some off-campus entities with NIH funding, for their basic research projects or as part of basic research collaborations with other academic institutions or nonprofit organizations, which do not include involvement or funding from for-profit (commercial) organizations. The CTAC reserves the right to refuse service to any entity or project that may present a biological or health hazard, introduce potential pathogens, or otherwise pose a risk to staff or contamination to the facility.