For each use of this instrument, you will need to bring the following items:
The CTAC reduced prices for all instruments’ use in 2015 for the benefit of the user base. The provided resources are limited to PPE, 70% ethanol, BioClean™, heat pads, fill tubes for isoflurane and towels. The following list are items recommended by the CTAC to facilitate your use of the instrument.
Animals’ eyes have no blinking or nictitating function under anesthesia, and are prone to drying out across their surface with anesthesia lasting over 15 minutes.
CTAC recommends Optixcare™, a veterinary product specifically for providing eye lubrication for routine protection provided to eyes during anesthetic. This product is an economical and effective tool for use as part of the multi-pronged approach to dealing with “dry eye”.
The key ingredient in Optixcare™ Eye Lube is the carbomer gel. Carbomer gel brings a viscosity to the gel which causes it to remain on the cornea for an extended period of time. This viscosity allows for control of the drop applied which makes it easy to apply. Optixcare™ Eye Gel becomes more liquid immediately on contact with the salt in the tears of the eye. Unlike mineral oil or petroleum based eye ointments, Optixcare™ Eye Lube is formulated with a sterile water base. Because of the water base and the gel viscosity only one drop per eye of Optixcare™ Eye Lube is needed.
Isoflurane scrubbers are used to recover expended waster anesthetic vapor. Two are required for the operation of the IVIS Spectrum, and area safety measure to protect operators.
Isoflurane scrubbers for this instrument come as activated charcoal filter canisters. These must be weighed to record initial weight, and re-weighed at the completion of each use to measure the accumulated weight of waste bound to the activated charcoal. The canister must not be used after a gain of 30 grams.
Isoflurane vapor anesthetic is the anesthetic of choice for use in the instrument. The instrument vaporizer will have a high and low mark on the isoflurane reservoir. Upon start of your session, please ensure that this is at the high mark. If it is not, please contact CTAC personnel to appraise them of the level status. CTAC provides two isoflurane filler tubes in the instrument room L5-109 for client use. After the imaging session, the client will refill the isoflurane reservoir on the instrument vaporizer to the high mark before leaving the room.
Not required, but sometimes very helpful is an exfoliant. Dependent on fluorochrome emission and signal strength, the animals’ hair may interfere with signal acquisition. Shaving and / or a depilatory cream may be used to denude the area to be scanned to mitigate signal loss.
Nair™ Hair Remover – this is known for its depilatory action that works by breaking the disulfide bonds of the keratin molecules in hair. This reduces the tensile strength of the keratin so greatly that the hair can be wiped away. Two of the active ingredients are calcium hydroxide (lime) and sodium hydroxide (lye), which raise the pH of hair, chemically breaking it down. Some formulations also contain potassium thioglycolate, which breaks down the disulphide bonds in the hair’s keratin thus weakening it enough to be simply wiped away. Products such as Nair™ often combine softening agents such as mineral oil to help offset the harsh active ingredients.