CTAC / ICBR SOP : Recognition of Components of the Software Control Panel
Title: Recognition of Components of the IVIS Software (Living Image™) Control Panel
- Living Image™ software
To familiarize the user with the components of the IVIS system’s software control panel.
Software component identification:
This is the control panel. It has been split into 6 functional zones. The camera settings will be covered in SOPs CTAC / ICBR SOP Camera Control During a Fluorescent Scan or CTAC / ICBR SOP Camera Control During a Luminescence Scan
Area A. This area is used to control the vertical focal plane relative to the camera. There are only 2 areas for user control. All others are for CTAC staff only, in order to make major changes or conduct maintenance activities.
1. Users may select or change the value in “Field of View”. Displayed here, it is on the default setting, C. The options are A, B, C, D and E, all of which correspond to different shelf heights within the cabinet as the instrument is being used to acquire data. As the system camera is in a fixed position, it is necessary to move the shelf to different heights to “”zoom” the picture in or out (see graphic).
2. Users may alter the “Subject height” value to correspond to the highest (tallest) animal in a scanned group, or the highest point on a single experimental subject. This ensures that the scan captures the entirety of the signal from the experimental subject.
Area B & C. This area encompasses controls for the black and white picture that your scanned fluorescence or luminescence signal is overlaid upon. All settings are optimized, and should not be changed.
Area D. This area is the toggle control for the user to select either a Fluorescent scan, or a Luminescent scan. The default is toggled to a Luminescent setting. To the left is the default color table that is used for each.
Area E. This is the system status area, and requires no user input. In the larger text box under the letter E there will be system messages that come up during an image acquisition if there are aspects to the settings that need addressing (e.g. saturated pixels) or that are parameter changes initiated by the instrument to move it to a more sensitive state to attempt to acquire a weaker signal than the input settings would allow. When system messages appear here, they are duplicated in a log window that will appear towards the bottom of the screen. Scrolling back the log messages will allow the user to see the saturation events or see what changes to the parameters were initiated by the instrument.
The temperature bar refers to the camera in the instrument, which is cooled to -90oC. This should be green at all times. If this is not the case, this should be brought to the attention of CTAC staff immediately.
Area F. Here are the major instrument controls. The Acquire button initiates a scan with the parameters inputted into the camera controls next to the Fluorescent / Luminescent toggle controls. When in Sequence Mode, this button will display Acquire Sequence instead. This control starts the instrument scan to generate your picture. The Imaging Wizard button is a software Wizard to assist in setting up your parameters. The >>Sequence Setup button is the toggle for changing from a single picture acquisition to a multiple picture acquisition. Initialize is the button to perform initialization of the instrument, where it will self-check / perform diagnostics and make sure all the filters, motors and servos are working correctly. This procedure is mandatory before and after using the instrument.